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Chromatin states were investigated in Drosophila cells by looking at the binding location of proteins in the genome.
Use of ChIP-sequencing revealed regions in the genome characterised by different banding.
Different developmental stages were profiled in Drosophila as well, an emphasis was placed on histone modification relevance.
A look in to the data obtained led to the definition of chromatin states based on histone modifications.
The human genome was annotated with chromatin states.
These annotated states can be used as new ways to annotate a genome independently of the underlying genome sequence.
This independence from the DNA sequence enforces the epigenetic nature of histone modifications.
Chromatin states are also useful in identifying regulatory elements that have no defined sequence, such as enhancers.
This additional level of annotation allows for a deeper understanding of cell specific gene regulation.
H3K23ac and the oncoprotein TRIM24 are higher in HER2-positive breast cancer patients and is a poor prognosis.
The histone mark acetylation can be detected in a variety of ways:
1.
Chromatin Immunoprecipitation Sequencing (ChIP-sequencing) measures the amount of DNA enrichment once bound to a targeted protein and immunoprecipitated.
It results in good optimization and is used in vivo to reveal DNA-protein binding occurring in cells.
ChIP-Seq can be used to identify and quantify various DNA fragments for different histone modifications along a genomic region.
2.
Micrococcal Nuclease sequencing (MNase-seq) is used to investigate regions that are bound by well positioned nucleosomes.
Use of the micrococcal nuclease enzyme is employed to identify nucleosome positioning.
Well positioned nucleosomes are seen to have enrichment of sequences.
3.
Assay for transposase accessible chromatin sequencing (ATAC-seq) is used to look in to regions that are nucleosome free (open chromatin).
It uses hyperactive Tn5 transposon to highlight nucleosome localisation.
Kyoto Gyoen National Garden
Kyoto Gyoen National Garden is a national garden of Japan.
It is situated around the Kyoto Imperial Palace.
Nobody's Wife (song)
"Nobody's Wife" is the second single released from Dutch singer-songwriter Anouk's debut studio album, "Together Alone".
The song was written by Anouk, Satindra Kalpoe and Bart van Veen, and it was produced by George Kooymans, Barry Hay and John Sonneveld.
It was released through Dino Music in the Netherlands and Ireland, BMG in Europe and Australia and Columbia Records in the United States.
First issued as a single in the Netherlands, "Nobody's Wife" became a hit, reaching number two on both the Dutch Top 40 and the Single Top 100 charts.
In early 1998, the song began to gain popularity in the rest of mainland Europe, especially in the Flanders region of Belgium and the Nordic countries, reaching the top 10 in these regions; in Iceland, it peaked at number one for two weeks.
A music video shot in black and white was made for the song, featuring clips of Anouk singing and dancing to the song.
"Music & Media" wrote about the song: "This is an excellent rock ballad by the Golden Earring endorsed Dutch debutante artist (...).
Anouk has made a bold—and as it turns out, convincing—move into the territory already occupied by the likes of Alanis Morissette and Meredith Brooks."
The song was first released in the Netherlands in the latter half of 1997.
It first charted on the Single Top 100 chart on 20 September 1997 at number 94, then debuted on the Dutch Top 40 Tipparade chart on 4 October at number 30.
On 15 November, the song rose to number two on both charts on stayed at that rank for four weeks on both listings.
It remained on the Dutch Top 40 for 20 weeks and the Single Top 100 for 40 weeks, earning a Platinum certification from NVPI for shipments of over 75,000 units.
According to the Single Top 100 chart, it was the ninth-best-selling hit of the year.
After its Dutch success, "Nobody's Wife" began to receive airplay throughout western and northern Europe.
It made its first foreign chart appearance on the Swedish Singles Chart on 12 December 1997, climbing to a peak of number two on 16 January 1998 and keeping the position for five weeks.
It ended 1998 as Sweden's 17th-most-successful song and went Platinum for shipping over 30,000 copies.
The single is also certified Platinum in Norway, where it peaked at number two for three weeks.
In the Flanders region of Belgium, "Nobody's Wife" reached number five on the week of 28 February and eventually went Gold for sales exceeding 25,000, and in Denmark, it reached number six in late January.
In February 1998, "Nobody's Wife" charted in Finland, rising to number nine three weeks after its debut.
The song then garnered success in Iceland starting in early March, debuting at number four on the Íslenski listinn chart and topping the listing on the weeks of 20 and 27 March.
A few months later, on 27 June, the single debuted on the French Singles Chart at number 80.
It reached its peak of number 34 on 8 August and spent a total of 14 weeks in the top 100.
The song was released in the United States in September 1998 but failed to make a significant impact, charting only on the "Billboard" Heritage Rock chart at number 32.
In March 2010, the single appeared on the German Singles Chart for only one week, at number 87.
Dutch CD single/Irish CD promo
European maxi single 1/Australian CD single
European maxi single 2
French/German CD single
US CD promo
US 7" single
New Jersey Coalition for Vaccination Choice
The New Jersey Coalition for Vaccination Choice (NJCVC) is a state-level anti-vaccination group advocating against mandatory vaccination.
Since its foundation in 2008 by Sue Collins and Maureen Drummond, the group's spokespersons have been advocating against mandatory vaccination for school children.
While they reject the "anti-vaccine" label, they repeat messages common to other anti-vaccination groups about the alleged lack of testing of multiple vaccines, while denying vaccination was effective in eliminating infectious diseases such as polio.
Scientists and medical experts have countered many of these statements, arguments against vaccination being contradicted by overwhelming scientific consensus about the safety and efficacy of vaccines.
Several experts such as Paul Offit have said campaigns by groups such as the New Jersey Coalition for Vaccination Choice contribute to vaccine hesitancy, even as New Jersey's vaccination rates decline, putting the population in increasing danger.
New Jersey Coalition for Vaccination Choice was instrumental to bringing some 400 protesters to the New Jersey State House on December 12, 2019, as legislators were debating a measure meant to tighten the state's vaccine exemption regulations.
Supported by anti-vaccination activist Del Bigtree, some of the parents said they would take their children out of school rather than have them vaccinated.
A call for action by the group brought an angry crowd to committee hearings in 2018 when state legislator were debating a similar measure.
A 2009 meeting between representatives of the group, including anti-vaccination activist Louise Kuo Habakus, and New Jersey governor Chris Christie put the governor in political difficulty when he seemed to agree with the debunked belief that vaccines cause autism.
John of Würzburg
John of Würzburg (Latin "Johannes Herbipolensis") was a German priest who made a pilgrimage to the Holy Land in the 1160s and wrote a book describing the Christian holy places, the "Descriptio terrae sanctae" (Description of the Holy Land).
All that is known of John's life is what he records in his "Descriptio".
He says that he was a priest of the church of Würzburg and he dedicated his work to a friend named Dietrich (Theoderic).
The Tegernsee manuscript calls John the bishop of Würzburg, but there was no bishop named John.
Possibly the copyist or whoever added the description of John to the Tegernsee manuscript confused him with his friend, who is sometimes identified with Dietrich of Hohenburg, who was bishop of Würzburg in 1223–24.
This identification is not certain.
Nor is the identification of Dietrich with the man of the same name who went on a pilgrimage around 1172 and wrote his own account of it, the "Libellus de locis sanctis".
John's pilgrimage took place while the holy places belonged to the Christian Kingdom of Jerusalem, but before the major renovation of the Church of the Holy Sepulchre.
He may have written his "Descriptio" several decades after the pilgrimage, possibly after 1200.
His account is not entirely based on what he himself saw, he admits that he made use of eyewitness reports and in some cases borrowed from other travel guides (especially Fretellus).
He probably landed at Acre, when he travelled to Nazareth, Jenin, Nablus, Jerusalem, Bethlehem and Jaffa, where he took ship home.
His description of these places is mostly that of an eyewitness.
The "Descriptio" is known from four manuscripts.
The earliest and longest, now Clm.
19418 in the Bayerische Staatsbibliothek in Munich, dates to the late 12th or early 13th century and comes from Tegernsee Abbey.
John's Latin is educated but ordinary.
John's purpose in writing was to update the 7th-century description of the Holy Land, "De locis sanctis", which he knew from the version edited by Bede, based on the construction projects that had taken place since the First Crusade.
The text is structured around the life of Jesus and divided into seven sections highlighting his birth, baptism, passion, descent into Hell, resurrection, ascension and judgement.
This structure was considered irrational by Titus Tobler, who rearranged the text for his edition.
The "Descriptio" contains information about the different Christian denominations in the Kingdom of Jerusalem.
It has also aroused interest for its early indications of the rise of national feeling in Europe.
John was a German patriot who laments the lack of credit given to the German crusaders.
In his thirteenth chapter, he writes:
Three days afterwards is the anniversary of noble Duke Godfrey [of Bouillon] of happy memory, the chief and leader of that holy expedition, who was born of a German family.
His anniversary is solemnly observed by the city with plenteous giving of alms in the great church, according as he himself arranged while yet alive.
But although he is there honoured in this way for himself, yet the taking of the city is not credited to him with his Germans, who bore no small share in the toils of that expedition, but is attributed to the French alone.
Paul Ayongo
Paul Ayongo (born 16 November 1996) is a Ghanaian professional footballer who plays as a forward for Mafra, on loan from Paços de Ferreira.
Ayongo made his professional debut with Paços de Ferreira in a 3-2 Taça da Liga win over Académico de Viseu on 28 July 2018.
They Call It Makin' Love
"They Call It Makin' Love" is a song written by Bobby Braddock that was originally recorded by American country artist Tammy Wynette and released as a single in 1979.
"They Call It Makin' Love" was first recorded in December 1978 at the Columbia Recording Studio in Nashville, Tennessee.
Additional tracks were recorded during this session, which would ultimately become part of Wynette's studio album released with the single.